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EWSR1 is required for HCV efficient RNA replication and viral protein production of a genotype 1b replicon. (A and B) A cell line stably expressing a HCV 1b Con1 replicon was transfected with the indicated siRNAs and maintained for 72 h. After 72 h, cellular RNA (A) or protein (B) was harvested. Replicon RNA was quantified using quantitative RT-PCR. Cellular protein was separated by SDS-PAGE and immunoblotted for NS5B or actin.
