Fig 5.

Cofractionation of ESWR1 with HCV replication complexes. Detergent-resistant membranes were prepared from Huh-7.5 cells with or without HCV replicons by Triton X-100 treatment at 4°C, followed by ultracentrifugation. Detergent-resistant (DR) membranes remain at the top of the sucrose gradient (fractions 1 to 3), whereas detergent-sensitive (DS) membranes sediment in the lower fractions (fractions 4 to 9). Protein lysates from pooled fractions were run on SDS-PAGE and probed for EWSR1, HCV NS5A, calnexin, and caveolin-2 (Cav-2).