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Validation of microarray by qPCR of selected genes. A subset of 10 genes was randomly chosen to confirm the microarray results by real-time PCR. Log ratios indicate changes observed in total (Tot) and polysomal (P) mRNAs during infection (compared to noninfected controls). The results of mRNA quantification by the two techniques were consistent. As expected, the fold changes were higher with qPCR.
