Table 1.
Chikungunya virus host range transmembrane deletion mutations and titers compared to those of SINV
| Mutant | Sequenced | Titer (PFU/ml) |
|
|---|---|---|---|
| BHK | C7-10 | ||
| SINV | |||
| HRa | 365VYTILAVASATVAMMIGVTVAVLCAC390 | 1 × 107 | 1 × 109 |
| ChikV | |||
| 37997b | 365TMTVVIVSVASFVLLSMVGTAVGMCV390 | 3 × 108 | 5 × 108 |
| TM17-1b | 365TMTVVIVSVASFVLLSMVGTAVGMCV390 | 7 × 105 | 2 × 106 |
| TM17-2b | 365TMTVVIVSVASFVLLSMVGTAVGMCV390 | 4 × 105 | 2 × 106 |
| TM17-3c | 365TMTVVIVSVASFVLLSMVGTAVGMCV390 | 1 × 105 | 1 × 106 |
a
HR, heat-resistant strain. The portion of the sequence in bold represents the segment of the TMD deleted in the original SINV TM-17 mutant.
b
Titers from 2 days postinfection.
c
Titers from 3 days postinfection.
d
The underlined portions of the sequences represent the segments of the TMD that were deleted.