Fig 7.

Knockdown of pig-tailed macaque BST2/Tetherin and replication of HSIV-vif clones. (A) PTM CD4 T cells were transduced with lentiviral vectors encoding BST2 shRNA, scrambled shRNA control, or control empty vector and the GFP⁺ cells enriched by cell sorting. The levels of PTM BST2 downregulation were assessed by flow cytometry and compared to those of untransduced cells. (B) Transduced PTM CD4⁺ T cells were infected with HSIV-vif-NL4-3 and HSIV-vif-Yu2 viruses at an MOI of 0.01 in the presence and absence of IFN. Viral supernatants collected at various days postinfection were assayed by HIV-1 p24 ELISA.