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L4-33K mutant virus infectivity and genome replication. (A and B) Ratios of physical particles to infectious particles of Ad5-WT and 33K− virus were determined in A549 cells (particle/FFU ratio) (A) and TetC4-33K cells (particle/PFU ratio) (B). (C) Viral genome replication in Ad5-WT-, or 33K−-infected A549 cells. The genome copy number was determined by quantitative PCR and normalized to the GAPDH value; this value was relative to that of Ad5-WT at 6 hpi.
