Fig 6.

ChIP assays for packaging protein binding to the PS in vivo. A549 cells were infected with Ad5-WT or 33K⁻, and L4-22K-, L4-33K-, IVa2-, L1-52/55K-, and IIIa-specific antibodies were used for ChIP to quantify binding to the PS. The results are presented as normalized fold enrichment by dividing the copy number of PS pulled down specifically by the antibody-antigen complex to the copy number of the E4-ORF6 fragment that was pulled down nonspecifically by the antibody. The fold enrichment value of each antibody was normalized to that of the preimmune serum negative control.