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. 2013 Jun;87(12):6635–6643. doi: 10.1128/JVI.00044-13

Fig 1.

Fig 1

(A) Generation of recombinant P2/P18 L segment fragment swapping mutants. Based on available unique restriction enzyme sites, 3 fragment swapping mutants were generated, replacing a fragment of the P18 L segment with the corresponding sequence of the P2 genome. Sequence changes are highlighted in black, and changes that result in amino acid differences between the two strains are numbered. (B) Mortality of guinea pigs infected with L segment P2/P18 fragment swapping mutant recombinant viruses. The mortality rate was defined as the number of animals reaching terminal points (>30% weight loss compared to a nomogram or rectal temperature of <38°C in addition to a marked decrease in the weight of the animal) within the course of an 18-day infection. Numbers of infected animals are as follows: rP18, n = 45; rP2, n = 24; rS18L18(A2), n = 8; rS18L18(C2), n = 3; and rS18L18(D2), n = 8. (C) Viremia levels of guinea pigs infected with L segment P2/P18 fragment swapping mutant recombinant viruses. Serum samples collected at terminal points (TP) from animals infected with rS18L18(D2), rS18L18(C2), or rS18L18(A2) were analyzed by plaque assay to determine viral titers. Each data point represents one animal: rP18, n = 29; rP2, n = 12; rS18L18(A2), n = 3; rS18L18(C2), n = 3; and rS18L18(D2), n = 6. dpi, days postinfection. *, P < 0.05; **, P < 0.01; ***, P < 0.001.