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Real time RT-PCR analysis of genome replication in Vero cells infected with recombinant viruses. Vero cells were infected at an MOI of 1 with virus, RNA was collected, and RT was performed with a primer specific for the 3′ end of the genomic S segment. Real-time RT-PCR was performed with primers specific to the NP gene, and values were normalized with values for GAPDH transcripts. *, P < 0.05; **, P < 0.01; ***, P < 0.001.
