Fig 4.

LANA and KDM3A recruitment sites on the KSHV genome. (A) LANA and KDM3A occupancy on the KSHV genome was examined by tiling array analyses. Co-occupying regions with more than 5-fold enrichment of both proteins are marked with orange shading. (B) Confirmation of ChIP-on-chip analyses. qPCR was performed on the samples with independent ChIP analyses. LANA and KDM3A were recruited to LANA loci but not the ORF64 coding region, which inversely correlated with the histone H3K9me2 mark. (C) Effects of LANA knockdown on KDM3A recruitment. Efficacy of LANA knockdown was examined by immunoblotting (a), and effects on viral copy number were monitored before ChIP analyses (b). Occupancy of LANA and KDM3A were examined by ChIP analysis followed by qPCR using primers for the LANA promoter and ORF64 coding region (c).