Fig 3.

Viral nonstructural proteins P5, P6, and P9-1 are the constituents of the matrix of the viroplasm induced by SRBSDV infection in VCMs. Shown is immunogold labeling of P5 (A), P6 (B), and P9-1 (C) in the matrix of the viroplasm in SRBSDV-infected VCMs at 84 hpi. Insets are the enlarged images of the boxed areas. Cells were immunostained for P5, P6, and P9-1 with P5-, P6-, and P9-1-specific antibodies in panels A, B, and C, respectively, as primary antibodies, followed by treatment with goat antibodies against rabbit IgG that had been conjugated with 15-nm-diameter gold particles as secondary antibodies. Black arrows, viral particles; red arrows, gold particles; GM, granular matrix; FM, filamentous matrix. Bars, 100 nm.