Fig 2.

BALF4, BLLF1, and BcLF1 are late (L) genes. (A) Lytic replication-induced tet-BZLF1/B95-8 cells were cultured in the absence or presence of PAA (phosphonoacetic acid) and harvested at 48 h postinduction. Total RNAs were purified using TriPure isolation reagent and applied for real-time RT-PCR as described in Materials and Methods. Levels of BMRF1, BALF4, BcLF1, and BLLF1 mRNAs were normalized to GAPDH mRNA levels. The value obtained in the absence of PAA was set to 100%, and data are expressed as means ± standard deviations (SD) from 3 biological replicates. dox, doxycycline. (B) Expression profiles for mRNA levels of EBV early (E) and L genes. Lytic replication-induced tet-BZLF1/B95-8 cells were harvested at 0, 24, and 48 h postinduction. Total RNAs were purified using TriPure isolation reagent and applied for real-time RT-PCR as described in Materials and Methods. Levels of BMRF1, BALF5, BALF4, BcLF1, and BLLF1 mRNAs were normalized to GAPDH mRNA levels. The value obtained at 0 h postinduction was set to 1, and data are expressed as means ± SD from 3 biological replicates.