Fig 4.

BcRF1, the EBV late gene transactivator, is located inside the BMRF1 core. Tet-BZLF1/B95-8 cells were transfected with HA-BcRF1 expression vector and then treated with doxycycline to induce lytic replication. Cells were harvested at 48 h postinduction, treated with mCSK buffer, fixed, and stained with anti-BMRF1 (green) and anti-HA (red) antibodies. (A) The data are presented as 3D surface reconstruction images of BMRF1 and HA-BcRF1 (left and middle panels). A merged 3D surface reconstruction image is also presented (right panel). (B) The data are presented as serial x-y, x-z, and y-z 3D surface reconstruction images (3D) and corresponding 2D images (2D). The 2D images show brightest-point sections in the x, y, or z axis.