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AgQ1 region recognized by the neutralizing Mab AgQ 1-1. 293T cells were transfected with wild-type or mutant AgQ1 with AgQ2, AgH, and AgL. The cells were harvested at 48 h posttransfection. (A) The cells were lysed and immunoblotted with the Mab AgQ1-119. WB, Western blotting. (B) The transfected cells were fixed and stained with Hoechst 33258 or with the Mab against AgQ1 (AgQ 1-1 or AgQ1-119) or AgQ2 (AgQ2B).
