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Confirmation of complex formation of HHV-6A gH/gL/gQ2 with wild-type or mutant AgQ1. Wild-type HHV-6A gQ1 or each mutant was cotransfected with AgH, AgL, and AgQ2. Forty-eight hours later, the cells were harvested, lysed, and immunoprecipitated (IP) with an anti-gH Mab, gH F5, followed by Western blotting (WB) with Mabs for AgQ1 (AgQ1-119), gH (gH1-1), gL (AgL4), and gQ2 (AgQ2B).
