Fig 6.

Interaction of AgH/AgL/AgQ1 (wild type or each mutant)/AgQ2 with CD46. 293T cells were cotransfected with AgH, AgL, AgQ2, and wild-type or mutant AgQ1, and 48 h later, the cell lysates were prepared and used for a CD46 binding assay. CD46 protein was isolated from 293T cells transfected with a CD46-expressing plasmid using an anti-CD46 Mab cross-linked to protein G-Sepharose. The Sepharose was then incubated with the cell extracts, shown in the left panels, and the eluates from the Sepharose were blotted with the indicated Mabs, shown in the right panels.