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. 2013 Jun;87(12):6911–6924. doi: 10.1128/JVI.02943-12

Fig 5.

Fig 5

Establishment of the superinfection model and Il22/Il22bp expression analysis. (A) Wild-type mice were infected with IAV (50 PFU) and, 3, 7, 14, or 28 days later, mice were challenged with S. pneumoniae (104 CFU). The numbers of live bacteria in the lungs were determined 24 h after S. pneumoniae infection. The results of one representative experiment out of two are shown (means ± the SEM, n = 5). (B and C) WT animals were infected with 50 PFU (B) or with 600 PFU (B and C) of IAV. The lungs were collected at 3 and 7 days p.i. (B) Il22 and Il22bp mRNA copy numbers were determined by quantitative RT-PCR. The data are expressed as the fold increased over the average gene expression in mock-treated mice (n = 6). (C) The ratios of Il22 to Il22bp (50 PFU versus 600 PFU) are depicted at days 3 and 7 p.i.