Fig 1.

Gamma-tubulin loses centrosomal localization in HFFF2 and Vero cells infected with HSV-1. (A) HFFF2 (left panels) or Vero (right panels) cells were infected with 1 PFU/cell of HSV-1 for the indicated times before fixation. Gamma-tubulin was visualized using mouse monoclonal antibody GTU-88 (green), and infected cells were visualized with the PTNC rabbit antibody (red). The nuclei were counterstained with DAPI. Arrowheads show centrosomal localization of gamma-tubulin. Asterisks indicate infected cells in panels also containing uninfected cells. Bars, 20 μm. (B) Western blot analysis of lysates obtained from cells infected for the indicated times. The progress of infection was visualized using the DM165 antibody directed against the major capsid protein VP5, gamma-tubulin levels were assessed using the GTU-88 antibody, and GAPDH levels were monitored as loading controls.