Fig 2.

Gamma-tubulin retains centrosomal localization in PK15 and Vero cells infected with PrV. (A) PK15 (left panels) or Vero (right panels) cells were infected with 1 PFU/cell of PrV for the indicated times before fixation. Gamma-tubulin was visualized using mouse monoclonal antibody GTU-88 (green), and infected cells were visualized with the 1702 antibody (red). The nuclei were counterstained with DAPI. Arrowheads show examples of centrosomal localization of gamma-tubulin. Bars, 20 μm. (B) Western blot analysis of lysates obtained from cells infected for the indicated times. The progress of infection was visualized using the anti-capsid 1702 antibody to show either VP5 or the two triplex proteins, gamma-tubulin levels were assessed using the GTU-88 antibody, and GAPDH levels were monitored as loading controls.