Fig 3.

Differential loss of centrosomal localization of pericentrin in Vero cells infected with HSV-1 or PrV. Vero cells were infected with 1 PFU/cell of HSV-1 or PrV for the indicated times before fixation. Pericentrin was visualized using rabbit polyclonal antibody HPA016820 (green), and infected cells were visualized with the mouse antibodies 11060 against HSV-1 ICP0 and a5 against PrV pUL25 (red). The nuclei were counterstained with DAPI. Arrowheads show centrosomal localization of pericentrin. Asterisks indicate infected cells in panels also containing uninfected cells. Bars, 20 μm.