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. Author manuscript; available in PMC: 2013 Jun 7.
Published in final edited form as: Nat Neurosci. 2012 Oct 28;15(12):1636–1644. doi: 10.1038/nn.3242

Figure 6.

Figure 6

Preventing apoptosis fails to rescue diaphragm innervation in Hox5MNΔ mutants. (a–d) Deletion of Bax increases Scip+ motor neuron numbers both in control and in Hox5MNΔ mice at e14.5 (P<0.001). However, in Hox5MNΔ Bax−/− mice the PMC still appears disorganized, despite rescue of Scip+ neuron numbers (d). (e–f) ALCAM expression is increased in Bax−/− mice while it is only slightly recovered in Hox5MNΔ Bax−/− mice at e14.5 (f). (g–h) Retrograde labeling of phrenic neurons with DiI at e18.5 shows that motor neurons innervate the diaphragm in Hox5MNΔ Bax−/− mice. (i–l) Deletion of Bax in a Hox5MNΔ background does not rescue the diaphragm innervation defects seen in Hox5MNΔ mice. Both control and control Bax−/− mice exhibit a stereotypical diaphragm innervation pattern (i,k) while Hox5MNΔ and Hox5MNΔ Bax−/− mice show similar defects in diaphragm innervation at e15.5 (j,l), as seen by neurofilament staining. (m–n) Scip+ and total Isl1/2+ neuron numbers in the different strains. Scale bars=25μm (a–h), 200μm (i–l). Error bars represent s.e.m.