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. Author manuscript; available in PMC: 2013 Jun 7.
Published in final edited form as: Connect Tissue Res. 2011 Dec 16;53(4):307–312. doi: 10.3109/03008207.2011.650301

Fig. 2.

Fig. 2

FPLC profile of Q-Sepharose chromatography for acidic proteins extracted from the mouse long bone. Protein extracts was separated and collected into continuous 120 fractions for biochemistry analyses. a: FPLC chromatogram for bone extracts from D452A-DSPP transgenic mice. b: FPLC chromatogram for bone extracts from wild type mice. Western immunoblotting and Stains-All staining illustrated that the fractions between the two arrows (fractions 20 to 58) in(a) contained DSPP and its fragments, DSP and DPP.