Table 1.
Primary antibodies used in this study.
| Antibody | Source | Immunogen | Dilution |
|---|---|---|---|
| c-Fos | Santa Cruz Biotechnology, sc-7202, rabbit polyclonal | Amino acids 210–335 of human c-Fos | 1:2000 |
| PACAP | Gift from Jens Hannibal, Mab JHH1, mouse monoclonal | Rat PACAP-38 conjugated to ovalbumin by glutaraldehyde | 1:20 |
| VIP | Peninsula Laboratories, T-5030, guinea pig polyclonal | Synthetic VIP: H-His-Ser-Asp-Ala-Val-Phe-Thr-Asp-Asn-Tyr-Thr-Arg-Leu-Arg-Lys-Gln-Met-Ala-Val-Lys-Lys-Tyr-Leu-Asn-Ser-Ile-Leu-Asn-NH2 | 1:200 |
PACAP, pituitary adenylate cyclase-activating peptide; VIP, vasoactive intestinal peptide.