Table 2.
Primer Sequence
|
Gene |
Primer Sequence |
Position |
| Rat | ||
| GCLC; NM_012815.2 | Forward: GTGGACACCCGATGCAGTAT | 1708–1727 |
| Reverse: TCATCCACCTGGCAACAGTC | 1795–1776 | |
| Nrf2; NM_031789 | Forward: CTCTCTGGAGACGGCCATGACT | 549–570 |
| Reverse: CTGGGCTGGGGACAGTGGTAGT | 694–673 | |
| Keap1; NM_057152 | Forward: TGGGCGTGGCAGTGCTCAAC | 1465–1484 |
| Reverse: GCCCATCGTAGCCTCCTGCG | 1659–1640 | |
| β-actin; NM_0311442 | Forward: CCTCTATGCCAACACAGTGC | 957–976 |
| Reverse: CATCGTACTCCTGCTTGCTG | 1171–1152 | |
| Bovine | ||
| GCLC-ARE4; NC_007324 | Forward: ACGCAGGCTCTCCCAGGTTGA | −4185 to −4164 |
| Reverse: ACATCTCATCCGGCCCTGCAG | −3898 to −3918 | |
| Nrf2; NM_001011678 | Forward: CTCCCAGGTAGCCCCCATTCCC | 397–418 |
| Reverse: CTGGGCTCTCGATGTGGCTGG | 554–534 | |
| GCLC; NM_001083674 | Forward: CCAGCACGTGGCGCATCTCT | 1008–1027 |
| Reverse: CAGCTGAACCTCCATGGGCCG | 1203–1183 | |
| Keap1; NM_001101142 | Forward: ATGAGCGTACCCCGCAACCG | 1313–1332 |
| Reverse: AGCCACTCCCACCCCGATCC | 1486–1467 | |
| β-actin; NM_173979 | Forward: CGCCATGGATGATGATATTGC | 89–109 |
| Reverse: AAGCCGGCCTTGCACAT | 154–138 | |
| Human | ||
| Nrf2; NM_001145412 | Forward: TCAGCCAGCCCAGCACATCC | 813–832 |
| Reverse: TCTGCGCCAAAAGCTGCATGC | 931–911 | |
| GCLC; NM_001197115 | Forward: GCAAGGCCCAGAACAGCACG | 1875–1894 |
| Reverse: TCCCTCATCCATCTGGCAACTGT | 2106–2084 | |
| β-actin; NM_001101 | Forward: AGCCTCGCCTTTGCCGATCCG | 34–54 |
| Reverse: TCTCTTGCTCTGGGCCTCGTCG | 270–249 | |