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. 2013 Apr;20(4):371–381. doi: 10.1177/1933719112459239

Figure 4.

Figure 4.

Expression profile of progesterone receptor (PR) and its target genes before and after human chorionic gonadotropin/luteinizing hormone (hCG/LH) treatment. A, Mice (n = 20) were subjected to superovulation as described in Materials and Methods sections. Ovaries were collected at 0 hours (without hCG), 4, 8, and 11 hours after hCG injection and the total RNA was isolated. Real-time PCR was performed to analyze the expression of PR, Bdnf, Socs3, and Cyr61. The level of Rplp0 was used as internal control to normalize the gene expression. The messenger RNA (mRNA) levels are expressed as mean fold induction ± standard error of the mean (SEM). B, Mice (n = 21) were subjected to superovulation as described in Materials and Methods section. Vehicle or ulipristal acetate (UPA) was administered intraperitoneally at 8 hours after hCG injection. Ovaries were collected at 11 hours after hCG injection and the total RNA was isolated. Real-time PCR was performed to analyze the expression of PR-regulated genes Bdnf, Cyr61, Errfi1, Runx1, Socs3, and PPARγ in response to vehicle and UPA treatments. The level of Rplp0 was used as an internal control to normalize the gene expression.