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In A, SDS-PAGE analysis of the S-200 fraction containing the purified QsdB. In B, degradation kinetics of OC8HSL and C6HSL in the presence of QsdB at 0.1 mg/ml; the protein-free buffer was used as a control. Three replicates were done. In C, Amidase activity of QsdB toward C6HSL releases hexanoic acid and homoserine lactone which was characterized and quantified by HLPC/MS as described in the material and method section.
