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. 2013 Jun 7;8(6):e66133. doi: 10.1371/journal.pone.0066133

Figure 5. Gα12 promotes OSCC cell invasive behavior via the regulation of IL-6.

Figure 5

(A) The Gα12-induced cell invasion of HSC-3 is suppressed by the treatment of cells with anti-IL-6 antibody. Representative images of the transwell invasion assays of the Gα12-overexpressing HSC-3 cells treated with or without anti-IL-6 antibody for 24 h (left panel). Quantification of invasion is shown in the right panel. (B) Supplementation of culture media with IL-6 restores cell invasion in Gα12-depleted cells. The Gα12-depleted HSC-3 and OC-3 cell lines were treated with or without three different concentrations of recombinant human IL-6 (0.5 ng/ml, 5 ng/ml, and 50 ng/ml) for 24 h prior to transwell invasion assays. The quantitative results of three independent experiments were analyzed by t-test. *P<0.05, **P<0.01, ***P<0.001. “ns” means no significance. Error bars represent SD of the mean from three independent experiments.