Table 1. Bacterial strains and plasmids.

Strain	Relevant Characteristics	References
Edwardsiella ictaluri
93-146	Wild type; pEI1+; pEI2+; Colr	[32]
EiΔfrdA	93-146 derivative; pEI1+; pEI2+; Colr; ΔfrdA	This study
EiΔgcvp	93-146 derivative; pEI1+; pEI2+; Colr; ΔgcvP	This study
EiΔglyA	93-146 derivative; pEI1+; pEI2+; Colr; ΔglyA	This study
EiΔsdhC	93-146 derivative; pEI1+; pEI2+; Colr; ΔsdhC	This study
EiΔmdh	93-146 derivative; pEI1+; pEI2+; Colr; Δmdh	This study
Escherichia coli
CC118 λpir	Δ(ara-leu); araD; ΔlacX74; galE; galK; phoA20; thi-1; rpsE; rpoB; argE(Am); recAl; λpirR6K	[33]
SM10 λpir	thi; thr; leu; tonA; lacY; supE; recA; ::RP4-2-Tc::Mu; Kmr; λpirR6K	[34]
S17-1 λpir	RP4-2 (Km::Tn7, Tc::Mu-1), ΔuidA3::pir+, recA1, endA1, thi-1,hsdR17, creC510	[35]
Plasmids
pMEG-375	8142 bp, Ampr, Cmr, lacZ, R6K ori, mob incP, sacR sacB	[36]
pEiΔfrdA	10242 bp, ΔfrdA, pMEG-375	This study
pEiΔgcvp	12231 bp, ΔgcvP, pMEG-375	This study
pEiΔglyA	14276 bp, ΔglyA, pMEG-375	This study
pEiΔsdhC	16295 bp, ΔsdhC, pMEG-375	This study
pEiΔmdh	18350 bp, Δmdh, pMEG-375	This study