Table 2. Primers with restriction enzyme used for the construction of the E. ictaluri mutants.
| Genes | Primer ID | Primer Sequence ((5′→3′)a | REb |
| frdA | EifrdAlflp | AAGAGCTCTCGTCCACTTCATTCATCAGAC | SacI |
| EifrdAlfrp | GTGGAAGTGGAAATCGAAAGA | ||
| EifrdArflp | TCTTTCGATTTCCACTTCCACGAAGCTCAGGAAGCCAAGAAG | ||
| EifrdArfrp | AATCTAGAGCAGGGAGATGATATTGAGGAC | XbaI | |
| EifrdA01S | CCTCAACTGAAGATTGCCTTA | ||
| gcvP | EigcvPlflp | AATCTAGACCTTTGGCGTGGAGATATGC | XbaI |
| EigcvPlfrp | AGCATCACTGTTTTCAAGCTG | ||
| EigcvPrflp | CAGCTTGAAAACAGTGATGCTGTAAAGCGCCTGGACGATGT | ||
| EigcvPrfrp | AAGAGCTCCGGACAGAGACATACCACCAA | SacI | |
| Eigcvp01S | GGCCTTTTGGTATGATTTGC | ||
| glyA | EiglyAlflp | AAGAGCTCGGGCATGGGTCAGTGAATAC | SacI |
| EiglyAlfrp | CCACAGCTCGGTATCGTAATC | ||
| EiglyArflp | GATTACGATACCGAGCTGTGGGTGAACGTCTTCCGGTCTATG | ||
| EiglyArfrp | AACCCGGGGCCTAGACGATGTCTCCTTGA | SmaI | |
| EiglyA01S | GGGCCAGATTTACTCAAAACC | ||
| sdhC | EisdhClflp | AAGAGCTCCAGCCTCCTTTGGTACTGCTA | SacI |
| EisdhClfrp | GCAAATCCAGATTGACAGGTCT | ||
| EisdhCrflp | AGACCTGTCAATCTGGATTTGCGGGTATGGTAAGCAACGCATC | ||
| EisdhCrfrp | AACCCGGGCCCCATCATGTAGTGACAGGT | SmaI | |
| EisdhC01S | CTCAGTCTCGTGGGATTTGC | ||
| mdh | Eimdhlflp | AAGAGCTCGGCTTTATAATGGCGTGTGG | SacI |
| Eimdhlfrp | AGGCAGCTGAGTCTTAAGCAG | ||
| Eimdhrflp | CTGCTTAAGACTCAGCTGCCTCTGGGCGAAGACTTTATCAAT | ||
| Eimdhrfrp | AACCCGGGGGAGCAGGCCCTACAAGACT | SmaI | |
| Eimdh01S | CAGCTCGCAATCTGAGTGTT |
a
RE: restriction enzyme sequence added to the 5′ end of the primer sequence.
b
Bold letters at the 5′ end of the primer sequence represent RE site. AA nucleotides were added to the end of each primer containing a RE site to increase the efficiency of enzyme cut. Underlined bases in internal primer (rflp) indicate reverse complemented internal primer (lfrp) sequence.