Skip to main content
. 2013 Jun 7;8(6):e66651. doi: 10.1371/journal.pone.0066651

Figure 1. PD98059 inhibits ERK1/2 phosphorylation in 17-day-old rats submitted to early weaning.

Figure 1

Animals were treated with 0.5% DMSO (control) or PD98059 at 300 µg/kg. Immunoblots and the respective densitometries are shown for p-ERK1/2, ERK1, p-Src and Src. Thirty µg of total protein were applied to each lane and each band is representative of one animal. β-actin was used as loading control. Relative densitometries as ratio of p-ERK/ERK1 and p-Src/Src are shown as fold change of control group and are presented by bars as means±SD (n = 3/group). *p<0.05 when comparing PD98059-treated group to control. Assays were conducted in duplicates.