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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Nat Med. 2012 Sep 9;18(10):1575–1579. doi: 10.1038/nm.2897

Figure 3.

Figure 3

Molecular basis of Sln-mediated thermogenesis. (a,b) Core body temperature after acute cold exposure and after dantrolene treatment in WT mice (a) and Sln−/− mice (b) with or without iBAT. n = 8 (WT + iBAT), n = 8 (WT – iBAT), n = 11 (Sln−/− + iBAT). (c) Alignment of the mouse Sln and Plb protein sequences. The amino acid residues Asp30 in Plb and Glu7 in Sln were mutated to cysteine for crosslinking experiments with Serca1a. (d) Western blot analysis showing Sln crosslinking to Serca1a in the presence of Ca2+ (0 –100 μM). (e) Western blot showing crosslinking of Plb with Serca1a in the presence of Ca2+ (0 – 100 μM). All data are means ± s.e.m.