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. 2013 Jun 7;8(6):e66236. doi: 10.1371/journal.pone.0066236

Figure 5. Identification of nfxB mutants using a luminescence-based reporter.

Figure 5

(A) Colonies of the mutT strain selected at 1.0 µg/mL Cip after 48 h of incubation. Examples of colonies with basal (non-mutated) and increased (nfxB mutants) luminescence are indicated (horizontal and vertical arrows, respectively). (B–C) Colonies of the mutT strain selected at a subinhibitory Cip concentration (0.4 µg/mL) after 24 h (B) and 56 h (C) of incubation. Detection of a subpopulation of cells with increased luminescence arising from colonies with basal luminescence indicates the emergence of nfxB mutants (examples are indicated by arrows).