Figure 8. Osmotin signaling mediated by adiponectin receptors induces suppression of gene expression via stress responsive promoter elements.

Cells (about 10⁸/mL) of strain BWG1-7a Δizh2::Kan_MX co-transformed with pSTRE-lacZ(LEU2) and p426-GPD (Vec), p426-GPD-AdipoR1 (pAdipoR1) or p426-GPD-AdipoR2 (pAdipoR2) were treated with osmotin (4 µM) in YPD for 45 min at 30°C and β-galactosidase activity was measured at the end of the treatment period. The values represent the means ± SE of two independent experiments with 3 to 4 samples per experiment.