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. 2013 Jun 7;8(6):e64795. doi: 10.1371/journal.pone.0064795

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© 2013 Cheng et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Left: Schematic of Serum_STD processed with a 0.22 um filtration step to form Serum_FILT. Right: Histogram content of platelets and microparticles in stepwise processed plasma samples. X-axis is particle diameter and Y-axis is number of particles/mL. (B) Platelet and microparticle content (in thousand/µL) of serum samples as measured by particle counter and hematology analyzer. (B) Validation of selected miRNAs by individual qRT-PCR assay. Normalized CT difference from mean of all 7 samples (including 5 plasma samples) is shown. MicroRNAs are arranged as in Figure 3. Average fold difference between Serum_STD vs. Serum_FILT is indicated below.