. 2013 Jun 7;8(6):e65312. doi: 10.1371/journal.pone.0065312

Table 3. Primers and probes used for PPV4 and PPV5 detection.

Primers/probes	Sequence (5′-3′)	Position
PPV4F	GCATTGGTGTGTGTCTGTGTCC	1068–1089^a
PPV4R	TCCTCTTTCCCGTTTGTTTCAT	1750–1771^a
PPV4DF	GCATTGGTGTGTGTCTGTGTCC	1068–1089^a
PPV4DR	GTGGCACATTTGTACATGGGAG	1391–1412^a
PPV4-Probe	FAM-5′- CTCCGCGGGATGTGCTTACAATTTTCA -3′-BHQ	1097–1123^a
PPV5-Probe	CAL Fluor Orange 560-5′- ACTTTGGTGTTGAGGGACTTAGCTTTTTTGTAC -3′-BHQ	1292–1324^b

Nucleotide position according to PPV4 (GenBank accession no. GQ387500).

Nucleotide position according to PPV5 (GenBank accession no. JX896321).