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. 2013 Jun 7;8(6):e65221. doi: 10.1371/journal.pone.0065221

Figure 2. SAGA complex purification.

Figure 2

a) Tandem affinity purification of a strain containing SptC tagged with the TAP tag (Lane 1) and a strain with wildtype SptC (Lane 2). The gel regions that were purified are numbered, and the S. cerevisiae homologues of the SAGA complex components identified in A. nidulans by LC MS are shown on the right. b) Tandem affinity purification of the N-TAPsptC;MYCacdX;nkuAΔ strain grown in media containing either 1% glucose (Lane 1), 50 mM arabinose (Lane 2) or 50 mM sodium acetate pH 6.0 (Lane 3). LC-MS was performed for all three conditions in this experiment. c) Figure 2c shows one of a further two repeat experiments, designed specifically to determine whether the differences in staining intensity around 50KDa in lane 3 of Figure 2b were robustly repeatable, showing that the apparent differences in part b are an artifact.