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. Author manuscript; available in PMC: 2013 Jun 7.
Published in final edited form as: Oncogene. 2012 Jul 23;32(23):2836–2847. doi: 10.1038/onc.2012.308

Figure 5.

Figure 5

CPE forms a complex with Wnt3a and Fz1 but does not interrupt the Wnt–FZ complex. (a) HEK293T cells were co-transfected with F-CPE-GFP, HA-Wnt3a, HA-GSK-3β or HA-Dvl as indicated. Total cellular lysate was immunoprecipitated using an anti-HA antibody and analyzed by western blotting using an anti-HA and an anti-GFP antibodies. (b) HEK293T cells were co-transfected with Wnt3a and the different CPE constructs as indicated. IP was performed as described in a. (c) HEK293T cells were co-transfected with FLAG-Hfz1-CRD and the CPE constructs as indicated. IP was performed using an anti-FLAG antibody. (d) HEK293T cells were co-transfected with Wnt3a, FLAG-Hfz1-CRD and F-CPE-GFP as indicated. IP was performed as described in c.