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Current traces for effect of oxidation and reduction. Representative ACh-induced current traces recorded before and after DTT (10 mM, 2 min), Cu:Phen (100:200 uM, 2 min) and EGTA (2mM, 2 min) applications for wild-type and mutant receptors. Current traces during reagent application are not shown. The bars above the traces indicate the duration of application of ACh; the arrows indicate the application of the given reagent. Holding potential was −60 mV.
