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. 1986 Apr;6(4):1315–1319. doi: 10.1128/mcb.6.4.1315

Biochemical and functional analyses of a secreted H-2Ld molecule.

C Murre, K C Parker, C S Reiss, J P Driller, D C Wiley, S J Burakoff, J G Seidman
PMCID: PMC367644  PMID: 3491290

Abstract

A truncated H-2Ld gene was constructed by deleting the transmembrane and cytoplasmic exons. The truncated H-2Ld gene was introduced into mouse L cells using the thymidine kinase gene as a selectable marker. Transformants were isolated and screened for the presence of truncated H-2Ld antigen. The truncated H-2Ld gene product was present in both the cytoplasm and culture medium, but not on the cell surface. The truncated H-2Ld antigen was stable in culture medium for at least 9 h and was secreted into the medium at a rate similar to the kinetics with which complete H-2 antigens reach the cell surface. Transformants expressing the truncated H-2Ld molecule were not recognized by cytotoxic T lymphocytes specific for the H-2Ld antigen.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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