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. 2013 Jun 7;8(6):e65330. doi: 10.1371/journal.pone.0065330

Figure 4. siE6AP mediated induction of adipogenesis is marked by increase in expression of proadipogenic factors.

Figure 4

(a) 3T3L1 cells were transfected with siE6AP or scrambled siRNA. Post 48 h transfection, cells were treated with MDI for until ten days; subsequently mRNA expression levels of indicated adipogenic genes was determined with qRT-PCR. Over expression of wild type E6AP down regulates while catalytically inactive E6AP-C843A up regulates proadipogneic factors: (b) 3T3L1 cells were transfected with E6AP and E6AP-C843A. After transfection cells were treated with MDI for 10 days and mRNA expression level of various adipogenic genes was determined with real time PCR. The primers used for C/EBPα, PPARγ, Leptin, SREBP1c and Adipsin listed in table 1.