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Apo-IscR does not bind a type 1 site containing symmetrical cytosines at positions 7 and 8 of each half site.
Binding isotherms of [2Fe-2S]-IscR (filled circles) and apo-IscR (open circles) with the iscRBcc site (5'-AAATAGCCGACCATTTTACTCGGGAATGTC-3', mutated dinucleotide is bolded, symmetrical positions 7 and 8 are underlined). Error bars represent the standard deviation of at least three separate assays.
