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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Nat Struct Mol Biol. 2013 May 5;20(6):740–747. doi: 10.1038/nsmb.2568

Table 3.

IscR-E43A does not discriminate for a cytosine dinucleotide at positions 7 and 8 in mutant hya sites

graphic file with name nihms-454929-t0001.jpg
a

The concentration of unlabeled mutant competitor required to decrease maximum binding (Amax) to labeled hya DNA by a factor of 0.5 (IC50).

b

Positions 7 and 8 are underlined.

c

The standard deviation is derived from fitting a four-parameter logistic curve to the average of at least three independent titration experiments.

d

Mutations in each competitor sequence are bolded.