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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Kidney Int. 2013 Feb 20;84(1):90–103. doi: 10.1038/ki.2013.43

Figure 2. Isoflurane increases CD73 activity, mRNA and protein synthesis in HK-2 cells.

Figure 2

Figure 2

Figure 2

Figure 2

Figure 2

A and B. HK-2 cells treated with isoflurane show time-dependent (A. N=6) and dose-dependent (B, N=4) increases in CD73 activity HK-2 cells were treated with isoflurane for 16 hr. Data are presented as means ± SEM. *P<0.05 vs. CD73 activity measured at baseline (A) or in cells treated with 0% isoflurane (B). Error bars represent 1 SEM. C and D. Representative images for CD73 mRNA (RT-PCR) and protein (immunoblotting) expression in HK-2 cells treated with isoflurane (Representative of 4 experiments). HK-2 cells were treated with 0–2.5% isoflurane for 6 hr (C) or with 1.25% isoflurane for 0–16 hr (D). Isoflurane caused dose (C) and time (D) dependent increases in CD73 mRNA and protein expression. E. Representative RTPCR images for CD73 protein (immunoblotting) expression in primary cultures of mouse kidney proximal tubule cells treated with carrier gas or with 2.5% isoflurane for 16 hr (Representative of 4 experiments).