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. 2013 Jun 7;8(6):e65260. doi: 10.1371/journal.pone.0065260

Table 2. BMAA concentration as determined by ELISA and LOD* (both expressed as µg/g DW) for unspiked extracts of sample 11 in two different solvents.

Solvent: sample diluent Solvent: water pH 7
Dilution LOD Replicate A Replicate B Replicate C Replicate D Replicate E Replicate F
1 0.6 n.a. n.a. n.a. n.a. n.a. n.a.
5 3 14.8 a.c. a.c. 17.6 25.6 31.0
10 6 n.d. 40.7 17.8 18.2 19.5 19.5
100 60 n.d. n.d. 76.4 n.d. n.d. n.d.
1000 600 n.d. n.d. n.d. n.d. n.d. n.d.
*

LOD: limit of detection, n.a.: not analysed, sample pH too low, a.c.: above calibration curve (equivalent to >300 µg/g BMAA in sample), n.d.: not detected.