Table 2. BMAA concentration as determined by ELISA and LOD* (both expressed as µg/g DW) for unspiked extracts of sample 11 in two different solvents.
Solvent: sample diluent | Solvent: water pH 7 | ||||||
Dilution | LOD | Replicate A | Replicate B | Replicate C | Replicate D | Replicate E | Replicate F |
1 | 0.6 | n.a. | n.a. | n.a. | n.a. | n.a. | n.a. |
5 | 3 | 14.8 | a.c. | a.c. | 17.6 | 25.6 | 31.0 |
10 | 6 | n.d. | 40.7 | 17.8 | 18.2 | 19.5 | 19.5 |
100 | 60 | n.d. | n.d. | 76.4 | n.d. | n.d. | n.d. |
1000 | 600 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |
LOD: limit of detection, n.a.: not analysed, sample pH too low, a.c.: above calibration curve (equivalent to >300 µg/g BMAA in sample), n.d.: not detected.