Figure 1. Physiological analysis of the salt-tolerant mutant mh31. (A) Phenotypes of Ws and mh31 (Ws background) ; after stratification, seeds were sown on the medium, grown and then photographed. Upper left and upper right panels, respectively, show Ws and mh31 grown on GM. Lower left and lower right panels, respectively, show Ws and mh31 grown on GM supplemented with 150 mM NaCl. (B−D) Greening rates of the plants grown on GM supplemented with the indicated concentration of NaCl (B), mannitol (C) or ABA (D). Stratified seeds were sown on the plate and incubated at 23°C for two weeks under continuous light (2,000 lx). After incubation, plates were photographed. The numbers of seedlings and greening seedlings were counted, and greening seedling rates were calculated. Data represent the average of values from quadruplicate plates of 20 seeds. Bars indicate standard errors. abi5-1 is in a Ws-2 background. Open circles, closed circles, open squares and closed square indicate Ws, mh31 (abi5-9), Ws-2 and abi5-1, respectively. Greening seedling rate is expressed as the percentage of seedlings with green cotyledons (n = 20). Mean ± SE; n = 4.