Figure 3. Interaction of abi5-9 with ABI3 in yeast two-hybrid system. Yeast strain PJ69-4 harboring constructs of the Gal4-Activation domain (AD) fusion and Gal4-DNA binding domain (BD) fusion, as indicated to the left and above, were grown for 4 d at 30°C on Synthetic Complete dropout medium lacking the amino acids leucine and tryptophan (SC-LW). Yeast cultures were replicated on the synthetic complete dropout medium lacking the amino acids leucine, tryptophan, histidine and adenine (SC-LWHA) with or without 3-amino-1,2,4-triazole (3-AT), and were grown for an additional 5 d (A) or 10 d (B) at 30°C. B1S, B1L and B1B2 are the partial regions of the ABI3, which interact with ABI5, as described by Nakamura et al.²⁷ Schematic representation of the full-length and truncated regions of ABI3 are indicated (B). A, B1, B2 and B3 are acidic domain, basic domain 1, basic domain 2 and basic domain 3, respectively. Numbers in parentheses indicate the position of amino acid residues from the first methionine of ABI3.