Figure 1. Effect of SoRI-20040, cocaine and Tat on the dissociation of [³H]WIN 35,428 binding from HEK/hDAT cells.

HEK293 expressing hDAT cells were incubated with [³H]WIN 35,428 (final concentration, 5 nM) for 2 h on ice. After the 2 h incubation, the incubation reagent was replaced with fresh assay buffer. At the zero time point, cocaine (10 μM) was added to condition-1 and condition-2 (see Table 2). Five minutes later, SoRI-20040 and Tat (5 μM) were added to condition-2 and condition-3, respectively. For the data analysis, the 100% control point (no drug) was time 0 for condition-1 and time 5 min point (no drug) for condition 2 and 3. Each data point is the mean ± S.E.M. (n = 3). * P < 0.05 (Student’s t test) compared with the cocaine condition.