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. Author manuscript; available in PMC: 2014 Apr 10.
Published in final edited form as: Chem Rev. 2013 Apr 10;113(4):2733–2811. doi: 10.1021/cr300354g

Table 3.

Traditional organelle-specific enzyme assays

Organelle Assay Reference
Autophagosome Acid phosphatase & cathepsin D Marzella, 1982160
Autophagosome Lactate dehydrogenase Kopitz, 1990161
Chloroplast Phosphoenolpyruvate carboxylase Uedan, 1976162
Chloroplast Chlorophyllase Arkus, 2006*163
Endosome Cathepsin H Claus, 1998164
Endosome Cathepsin S Claus, 1998164
Endosome Horseradish Peroxidase Pool Jr, 1983165
ER BADPH-Cytochrome C Reductase Sottocasa, 1967166
ER Glucose-6-Phosphatase Nordlie, 1966167
Golgi Thiamine pyrophosphatase Cheetham, 1971168
Golgi α-mannosidase II Pool Jr, 1983165
Lysosome Acid phosphatase Barret, 1977158
Lysosome β-galactosidase Pool Jr, 1983165
Lysosome β-hexosaminidase Barrett, 1977158
Lysosome Lysosome-specific dipeptidyl peptidase 1 Thong, 2011*169
Mitochondria Cytochrome c oxidase Storrie, 199085
Mitochondria INT reductase Pennington, 1961170
Mitochondria Succinate dehydrogenase Alston, 1977157
Nucleus Histone modifying enzymes Fischle, 2005171
Peroxisome Catalase Leighton, 1968159
Phagosome (Biotin-LC-Phe-Arg)2-Rhodamine 110 Yates, 2005172
Plasma Membrane 5'-nucleotidase Howell, 1982173
Plasma Membrane Alkaline phosphodiesterase I Touster, 1970174

Topic included in the manuscript are marked with an asterisk (*).