FIG. 2.

Relative fold expression of five different target genes, nuclear ubiquitous casein kinase and cyclin-dependent kinase substrate 1 (NUCKS1), CD59, S100A6, aryl hydrocarbon receptor nuclear translocator isoform 1/3 (ARNT1/3), and ADP-ribosylation factor-like 6 interacting protein (ARL6IP), expressed in the HMEC-1 cells incubated with anti-NS1 Abs (solid bar) compared to normal rabbit IgG incubated cells (open bar) and standardized against β-actin as the internal reference, were analyzed by real-time RT-PCR at 2, 4, 6, 8, 10, 12, 24, and 48 hours post incubation. Samples from each time point were tested in triplicate and the data represent the mean (±1 standard deviation) relative expression level was used for analysis that the target gene is downregulated, the same, or upregulated, respectively, compared with the control (*p<0.05; **p<0.01).