Figure 3.

l-Galactono-γ-lactone dehydrogenase (GalLDH) activity and expression level in the cultivars Vergasa and Biela of pepper (Capsicum annuum L.) fruits harvested at two temperature conditions (Harvest 1 and 2). (A) Activity determined spectrophotometrically. Column values with distinct letters are significantly different; (B) Analysis of the mRNA expression. Semiquantitative reverse transcription-PCR was performed on total RNA isolated from fruits of the two cultivars. A representative agarose electrophoresis gel of the amplification products visualized by ethidium bromide staining under UV light is shown. T/C indicates the relative level of the GalLDH amplification product (T) over the Actin (C, internal control) after normalization to the control samples and expresses the change in folds with respect to the corresponding controls (Actin from Harvest 1 in each cultivar). Values are means of at least three semiquantitative RT-PCR assays made in different fruits from each cultivar.